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A Multitude of Electrophoresis Applications in 1 System
A Multitude of Electrophoresis Applications in 1 System
The independent architecture of electrophoresis cells in a microplate strip offers significant advantages over conventional slab-gel based electrophoresis as each cell can have different internal designs and can contain gels, buffers, labeling reagents, and electrodes that are optimized for different applications.
The Axor 1 Box instrument interfaces with each electrophoresis cell independently from every other cell in a run, with real-time imaging feedback-based-control of each cell allowing each cell to be run with its own set of parameters.
The following applications are being developed on the system.
Analytical electrophoresis
Analytical electrophoresis
Analytical applications range from molecular weight analysis of DNA, RNA or Proteins with results equivalent to agarose or polyacrylamide gel electrophoresis.
In cells with capillary separation channels, high resolution molecular weight analysis can be performed on biomolecule fragments to viral particles and prokaryotic cells.
Preparative electrophoresis
Preparative electrophoresis
Preparative applications for the purification of sub-components from samples such as Next Generation Sequencing library preps or Synthetic Biology cloning reactions provide a precise and accurate method to extract defined sub-components from the mixture. These purified sub-components are removed from the electrophoresis cell and used for downstream reactions or applications.
Alternatively, a subtraction procedure can be used to remove unwanted materials from samples.
Affinity electrophoresis
Affinity electrophoresis
An exciting application of the electrophoresis-in-microplates system is to detect the presence of biomarkers, viruses, bacteria or cells in a sample based on their affinity to DNA probes, antibodies or aptamers.
DNA probes and antibodies are covalently immobilized in distinct ‘binding’ zones in the electrophoresis gel matrix. A labeled sample is introduced and electrophoresed through the gel matrix. RNA or DNA and antigens present in the sample are captured by the ‘binders’ and produce a semi-quantitative fluorescent signal in each zone.
The affinity electrophoresis-in-microplates format offers significant advantages:
No sample preparation: Purification process not required for most sample types.
Simple operation: User adds 1-8 samples into an 8-cell strip, places the strips into the instrument and presses START. Up to 96 samples (12 strips) with 8-cells) are processed in parallel.
Minimal infrastructure needs: The system uses a small instrument and ambient storage consumables. No additional devices are required.
Rapid testing: Results are produced in 15-30 minutes based on the level of multiplexy needed (higher plexy requires longer channels)
Robust interpretation: Tens to a hundred Genomic AND immunological biomarkers are detected in a single electrophoresis cell providing a more comprehensive interpretation based on multivariate data
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